Nitric Oxide Synthase (NOS) Activity Assay Kit II (Colorimetric)

MILPITAS, Calif. - July 28, 2021 - PRLog -- Nitric oxide synthases (EC 1.14.13.39, NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. NO plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes the five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS including endothelial (eNOS), neuronal (nNOS), and inducible (iNOS).

nNOS accounts for the production of NO in the central nervous system and participates in cell communication and information storage. eNOS produces NO in the blood vessels and is involved in the regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo during oxidative stress and large amounts of NO are produced as part of body's defense mechanism.

BioVision's Nitric Oxide Synthase Activity Assay Kit II provides an accurate and convenient method to assay NOS activity in a variety of sample types. In this assay, NO generated by NOS undergoes a series of reactions and then reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm. The assay is simple, fast, sensitive and is high-throughput adaptable.

Figures: (a) Nitrite Standard Curve. (b) Measurement of NOS Positive Control activity (10 µl). (c) Detection of endogenous NOS activity in J774.1A cell lysate (150 μg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma. Assays were performed following the kit protocol.

Storage Conditions -80°C
USAGE For Research Use Only! Not For Use in Humans.

For more information on this Assay Kit Cat# K2094 https://bit.ly/375J9op