XTT Cell Viability Assay Kit (Colorimetric)

MILPITAS, Calif. - Sept. 13, 2021 - PRLog -- BioVision's XTT Cell Viability Assay Kit is a colorimetric assay for studying cell proliferation and cell cytotoxicity. The assay is based on the reduction of the XTT tetrazolium salt by NADH, which is produced by the mitochondrial dehydrogenase enzymes of metabolically active cells to generate an orange-colored formazan product in the cell media.

This kit can be used for studying cell proliferation in response to cytokines, nutrients, growth factors and mitogens and also for analyzing the cytotoxic and cytostatic effects of drugs, toxins, antibodies and other exogenous chemicals. In this assay, the XTT working solution is added directly to the cell culture media and is read after an incubation of 0.5-4 hr.

The measured absorbance at 475 nm is proportional to the number of viable cells. There are no steps in the assay involving changing of media or washing or addition of any solubilization reagents. The assay can detect cell numbers as high as 500,000 viable cells and as low as 1000 viable cells in each well. The presence of phenol red and/or serum in the cell growth media does not interfere with the assay. The kit provides a safe, easy-to-use, non-radioactive, high-throughput method for characterizing and screening cell viability and cytotoxicity.

Figures. (A) Standard Curve of viable HeLa cells (0 to 500,000 cells/well). (B) Standard Curve of viable Jurkat cells (0 to 500,000 cells/well). (C) Cytotoxicity dose-response curve of L929 cells (2 x 104 cells/well) following 48 hr treatment with TNF-α (EC50 = 110.4 pg/ml
calculated by 4-parameter logistic curve fitting).

Storage Conditions -20ºC
USAGE For Research Use Only! Not For Use in Humans.
For more information on this Assay Kit Cat# K2108 https://bit.ly/3EfY2nF