Firefly Luciferase Assay Kit (Luminometric)

For determining the effect of inhibitors or inducers in in vitro cell-based assays and study of promoters and its interaction with transcription factors.
MILPITAS, Calif. - Dec. 9, 2020 - PRLog -- Luciferase-based assays are the most sensitive assays for studying gene regulation and function. Firefly luciferase is 100-fold more sensitive than the alternative reporters including chloramphenicol acetyltransferase, β-Glucuronidase and green fluorescent protein assays.

BioVision's Firefly Luciferase Assay Kit is designed for an accurate, sensitive and efficient quantitation of firefly luciferase reporter enzyme activity for studying gene reporter regulation and function. In this assay, firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescent system to produce light. It is designed to detect and quantitate firefly luciferase expression in mammalian cells. The kit ensures maximum sensitivity, consistency and strong bioluminescent signal.

Figure: Inducible promoter-driven luciferase expression in H1299 and HEK293 cells. 1x105 cells were cultured and treated with an inducible reagent for 16 hr. The cell lysates were then assayed for luciferase activity after incubation with the luciferase substrate for 1 min at RT.

For more information on this Assay Kit Cat# K2062, visit:

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