SARS-CoV-2 Pseudovirus BSL-1 Baculoviruses, by Montana Molecular

GÖTTINGEN, Germany - Sept. 16, 2020 - PRLog -- Pseudo SARS-CoV-2  enters the host cell through interactions with the ACE2 receptor and other host cell cofactors. Once inside the cell, the pseudovirus does not replicate, but delivers a genetically-encoded, fluorescent reporter that expresses bright red or green fluorescence in the host cell nucleus. When viral entry is successfully blocked, the host cell fluorescence decreases.

Two versions of Pseudo SARS-CoV-2supplied by Montana Molecular, US, are available. One carries the Spike protein originally sequenced from isolates obtained in Wuhan, the other carries D614G Spike. Both constructs have been sequence verified and tested in A549 cells. Pseudovirus affinity for the host receptor ACE2 was confirmed using absorption experiments on A549 cells expressing different amounts of ACE2.

Blocking Pseudovirus Entry

Pseudo SARS-CoV-2 is an enveloped virus that presents the full length spike protein on the viral surface membrane with the same topology as SARS-CoV-2. The spike protein is oriented perpendicular to the membrane such that multiple antibody binding sites are available and presented in a structurally relevant way.  Two versions are available, one with the sequence identified in the original Wuhan reference specimen and the newer D614G mutant that first appeared in Europe and is rapidly replacing the Wuhan strain.

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