- May 28, 2020
-- Beta-mannosidase (β-Man; EC 18.104.22.168) is an enzyme that hydrolyzes terminal, non-reducing beta-D-mannose residues in beta-Dmannosides. It is a member of the glycoside hydrolase family 2. There are numerous applications of β-Man including the synthesis of glycosides, production of fermentable sugars, curing mannosidosis, etc. Lysosomal β-Man is essential for catabolizing the Asn-linked glycans of glycoproteins thereby maintaining the cellular homeostasis. β-Man deficiency results in Beta-Mannosidosis, a rare inherited disorder affecting the break-down of mannose-containing disaccharides. These disaccharides gradually accumulate in the lysosomes and cause cells to malfunction. BioVision's Beta-Mannosidase Activity Assay Kit provides a facile, rapid way to monitor total β-Man activity in various biological samples. In this kit, β-Man cleaves a synthetic specific substrate and releases a fluorophore, which can be easily quantified (Ex/Em = 360/445 nm). The substrate is specific to β-Mannosidase and can differentiate from α-Mannosidase. The assay is simple, sensitive and can detect as low as 1.0 µU of beta-mannosidase activity.
Figures: (a). 4-Methylumbelliferone (4-MU) Standard Curve. (b). Measurement of purified β-Mannosidase (5 ng) and α-Mannosidase (6 ng) activities using BioVision's proprietary substrate. The kit can efficiently distinguish β-Mannosidase activity from α-Mannosidase. (c). βMannosidase activity in human plasma (10 μl) and 3T3 cells (2 μg protein). All assays were performed following the kit K2045 protocol.
For more information on this assay kit, visit: https://www.biovision.com/beta-mannosidase-activity-assay-kit-fluorometric.html