Acid Sphingomyelinase Assay Kit II (Colorimetric)

MILPITAS, Calif. - Jan. 25, 2019 - PRLog -- Sphingomyelinase (SMase) cleaves sphingomyelin to produce hosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger.There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B.
BioVision's Acid Sphingomyelinase Activity Assay Kit provides a simple
and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.

Figure:  ASMase activity of serum and macrophage cell lysate (1 µg/µl). Assays were performed following the kit protocol.

For detailed information on this assy kit, visit: https://www.biovision.com/acid-sphingomyelinase-assay-kit...