MILPITAS, Calif. -
Aug. 13, 2018 -
PRLog -- Phospholipids are major component of the bilayers of all plasma membranes. A single phospholipid molecule consists of a phosphate group on one ("head"), and two side-by-side chains of fatty acids that make up the "tails". The phosphate head groups can be modified with organic molecules such as Choline (Cho). Cho-containing phospholipids (Phosphatidylcholines;
PC) are critical for structural membrane integrity, cellular metabolism and signaling either as individual molecules or precursors of secondary messengers. Changes in global synthesis of Cho-containing phospholipids are an essential parameter in analysis of cellular response to both, physiological and pathological conditions, environmental stress, or drug treatment. To date, phospholipids biochemistry, cell biology and metabolism remain obscure, due to limited methods for their direct cellular visualization. BioVision's EZClick™ Global Phospholipid Synthesis Assay Kit (K537-100) offers a simple and robust method to label and visualize newly synthesized phospholipids
in vivo. Based on the metabolic incorporation of the choline analogs directly into their structure, modified phospholipid molecules can be detected with high sensitivity and spatial resolution by click chemistry with azide-containing dyes (Ex/Em= 494/521 nm). This kit enables quantitative analyses of global biosynthesis/
turnover of Cho-containing phospholipids in cells. Cells show strong incorporation of Cho analogs into all classes of phospholipids that can be assayed by microplate reader and fluorescence microscope. The kit provides sufficient materials for 100 assays.
Figure. Analysis of metabolic labeling of phospholipids in proliferating cells using K537-100. Jurkat cells (1X106 cells/well) were pre-treated with vehicle or cultured in presence of 1X EZClick
TM Phospholipid Label for 24 hours at 37°C prior to 1 hour treatment with Phospholipase D and then processed for detection of according to the kit protocol.
For detailed information on this assay, visit:
https://www.biovision.com/ezclicktm-total-phospholipid-as...
