Buffy Coat Extraction Using Plurispin & Pluribead

EL CAJON, Calif. - Jan. 14, 2023 - PRLog -- Preparation of the buffy coat is frequently the first stage of subsequent processing for T-cell depletion, tumor cell purging, or cryopreservation of both marrow and blood HPC collections.

Examining red blood cells is simple due to their abundance; thousands of red blood cells can be seen in a single drop of blood. White blood cells, on the other hand, are present in very small numbers and are more difficult to examine. A buffy coat smear examination is the quickest way to examine large numbers of white blood cells. The buffy coat is simply a collection of all of the white blood cells and platelets in a blood sample.

We will also find out how Plurispin Antibody Cell Separation technology is used in buffy coat extraction.

How should fresh whole blood be prepared for a Buffy Coat fraction?

Simply adhere to the brief protocol described below:

• Mix one part washing buffer with one part whole blood.
• While the brake is off, centrifuge the diluted whole blood for 10 minutes at 200 x g.
• Leukocytes in interphase should be removed (buffy coat)

A whole blood sample should always be handled with the same caution as if it were possible that it could transmit pathogens that cause infectious diseases. The time has come to separate the buffy coat from a whole blood sample after all the necessary preparations have been made.

Buffy Coat Extraction

The buffy coat is separated from the plasma and RBC by centrifuging the prepared whole blood sample. After centrifugation, there will be a thin layer between the RBC and plasma that accounts for about 1% of the sorted sample – the buffy coat. The experimenter uses a tiny pipette to gather and transfer the buffy coat to a different container.

How can Plurispin be useful?

A brand-new technique for isolating negative cells from whole blood, buffy coat, or cord blood is called the PluriSpin system. This new technique isolates live, unaltered, and highly purified cells in a single step without the use of magnets or a column. As a result, there is less chance of activating or harming the target cells.

How PluriBead can help?

The unique PluriBead cell separation technology does not use any magnetic components. The process is simple: Your pluriBeads are sieved through a strainer with your bound target cells on top, while the unwanted cells pass through. After detaching, you have your target cells ready.

For more information, you can visit our website. Utilize our products to see the difference for yourself. Get started with our cell separation products today!

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