Hyaluronidase Activity ELISA Kit

MILPITAS, Calif. - July 15, 2021 - PRLog -- Hyaluronidases, also known as hyaluronoglucosidases (HYALs) are family of enzymes that catalyse the degradation of hyaluronic acid (HA) by cleaving its glycosidic bonds. HA is a nonsulfated glycosaminoglycan and a major component of the extracellular matrix. It is involved in many biological processes including structural support, cell migration and tissue turnover. In humans, there are six known HYALs and are present in organs including spleen, skin, eyes, liver, kidneys, uterus, placenta etc. and in body fluids including saliva, tears, blood, semen etc.

They increase membrane permeability, and make tissues more readily permeable. However, HYALs have been also implicated in the progression of many solid tumor types including colorectal, bladder, prostate, breast and brain cancer. Additionally, HYALs have been used in ophthalmic surgery and dermatology.

BioVision's Hyaluronidase Activity ELISA Kit is a high throughput, quantitative immunoassay desgined to determine the activity of HYALs in various biological samples. In contrast to the traditional turbidity assay, this kit offers a simple, sensitive and convenient method to measure the activity of HYALs in biological samples. In this assay, samples are added to the wells of a 96-well plate pre-coated with biotin-HA. If the sample has any HYAL activity, it will bind to the biotin-HA on the plate and thus less free biotin-HA sites will be available following washing.

The biotin-HA is then detected using a specific streptavidin probe measured at OD 450 nm. The absorbance signal is inversely proportional to the HYAL activity in samples. The kit can detect as low as 0.5 mU of HYAL activity under the assay conditions.

Figures. A. HYAL Standard Curve. This Standard Curve is for demonstration only. B. Spike recovery experiment using human saliva and urine samples before and after spiked with 25 mU and 2 mU of HYAL respectively. Spike recovery was > 85%. C. HYAL activity determined in brain tissue using the assay conditions.

Storage Conditions -20ºC
USAGE For Research Use Only! Not For Use in Humans.

For more information on this Assay Kit Cat# E4909  visit: https://bit.ly/3rax9Lu