Cellulase Activity Assay Kit (Fluorometric)

A facile, rapid way to measure cellulase activity lower than 500 nU in various biological samples.
MILPITAS, Calif. - April 9, 2021 - PRLog -- Cellulases are enzymes that catalyze the decomposition of cellulose molecule into monosaccharides such as beta-glucose, or shorter polysaccharides and oligosaccharides. They are produced by fungi, bacteria, and protozoans. Cellulase is a complex of three different enzymes including β-1,4-endoglucanase, cellobiohydrolase, and β-glucosidase.

Cellulose is a major component of the plant cell wall and most mammals have a very limited ability to digest cellulose by themselves. So cellulose breakdown by fungal cellulases make a major constituent of plants available for consumption. Most fungal cellulases have a two-domain structure, a catalytic domain and a cellulose binding domain. Cellulase is used for commercial food processing in coffee. Additionally, cellulases are widely used in textile industry, laundry detergents, pulp and paper industry, pharmaceutical applications etc.

BioVision's Cellulase Activity Assay Kit provides a facile, rapid way to measure cellulase activity in various biological samples. In this kit, cellulase cleaves a synthetic substrate and releases the fluorophore, which can be easily quantified at Ex/Em = 365/450 nm. The substrate is specific for cellulase and can differentiate its activity from that of 1,3-β-D-glucanase. The assay is simple, sensitive and can detect cellulase activity lower than 500 nU in samples.

Figures. a. 4-MU Standard Curve, results from multiple experiments. b. Measurement of purified cellulase (2.5 ng) and 1,3-β-D-glucanase (2.5 ng) activities using BioVision's proprietary substrate. The kit can efficiently distinguish the cellulase activity from 1,3-β-D-glucanase. c). Cellulase activity in barley seeds (5 mg) and mushrooms (40 μg protein). All assays were performed following kit protocol.

For more information on this Assay kit, Cat# K2080, Visit: https://bit.ly/3d6rAIS

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