MILPITAS, Calif. -
Feb. 22, 2021 -
PRLog -- BioVision's Glycoprotein Staining Kit is designed for the staining and detection of glycoproteins on a PVDF or nitrocellulose membrane using the periodic acid-Schiff (PAS) method. The kit provides all the necessary reagents needed to specifically stain the glycoproteins separated by polyacrylamide gel electrophoresis and transferred to a nitrocellulose or PVDF membrane. The glycoproteins are detected as magenta bands against a colorless background. The kit contains sufficient reagents for staining up to 20 membranes (6 x 9 cm) and can detect as low as 0.5 µg of native glycoproteins.
Figures A. SDS-PAGE and Coomassie staining of various proteins. B. Selective staining of glycoproteins. Lanes, M: Protein Marker; 1: Deglycosylated recombinant glycoprotein;
2: Recombinant glycoprotein;
3: Positive control (native glycoprotein);
4: Negative control. C. Glycoprotein staining using various amounts of native glycoprotein (10, 5, 2.5, 1 and 0.5 µg respectively)
. For deglycosylation, 10 µg of glycoprotein (BioVision Cat# P1172) was treated with 0.1 mU of PNGase F (BioVision Cat# P1598) for 2 hr at 37 °C.
For more information on this Assay Kit Cat# K2073, Visit:
https://bit.ly/3bz4Jn0
