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Sulfadiazine ELISA Kit- A competitive enzyme immunoassay
For In vitro quantitative determination of Sulfadiazine concentration in serum, urine, etc.
By: BioVision, Inc.
BioVision's Sulfadiazine ELISA Kit is a competitive enzyme immunoassay for the quantitative analysis of sulfadiazine concentration in a variety of sample types. In this assay, the sulfadiazine antigen is pre-coated on the 96-well plate. The SDZ in the sample and the antigen on the plate compete for binding to the anti-sulfadiazine antibody. After the addition of the enzyme conjugate that binds to captured SDZ antibody, TMB substrate is added and color signal is developed. The OD value of the sample is inversely proportional to the SDZ concentration in the sample. This value is compared to the sulfadiazine standard curve and the SDZ concentration is subsequently obtained. The kit is simple, easy and a rapid way to determine sulfadiazine concentration. The kit can detect 4 - 256 ng/ml SDZ concentration in less than 1 hr (LOD 2 ng/ml).
Figures. A) Sulfadiazine Standard Curve. This Standard Curve is for demonstration only. B) Spike recovery experiment using human serum and urine samples spiked with Sulfadiazine (100 ng/ml) respectively. Spike recovery was 85-100%.
For more information on this ELISA Kit Cat# E4904, Visit: https://bit.ly/
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