Glutamine Synthetase Activity Assay Kit (Colorimetric)
A simple, plate-based colorimetric assay for measuring GS activity in biological samples.
By: BioVision Inc
BioVision's Glutamine Synthetase Activity Assay Kit is a simple, plate-based colorimetric assay for measuring GS activity in biological samples. In this assay, GS hydrolyzes Glutamate to Glutamine and ADP. ADP in the subsequent enzymatic reaction, in presence of ADP Converter, ADP Developer Mix, and ADP Probe forms a colorimetric product that is measured at OD 570 nm. The assay is simple, sensitive, and can detect as low as 10 µU of GS in biological samples.
Figures. a). ADP Standard Curve. b) Enzyme kinetics of rat brain extracts (1.3 µg). c) Glutamine Synthetase specific activity in rat brain (1.3 µg), rat liver (1.0 µg), and 3T3 cell lysates (1.0 µg). All assays were performed following kit K2056 protocols.
For more information on this kit, visit: https://www.biovision.com/