- Oct. 21, 2019
-- Phospholipase C (PLC) belongs to a family of membrane-associated enzyme that specifically cleaves the phosphoester linkage before the phosphate group in phospholipids. PLC catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into inositol 1,4,5- triphosphate (IP3) and diacylglycerol (DAG), which are second messengers critical for downstream signal pathways to control diverse cellular processes. PLC family consists of 13 isozymes split between six subfamilies. Members of the PLC family are involved in intra and inter cellular signal transduction pathways that are important for cell proliferation and differentiation. PLC isozymes have been also associated with several diseases such as cancer, inflammation, cardiovascular diseases and neuropathic pain. Therefore, investigation of PLC enzymes and their inhibition may lead to innovative therapeutic strategies. BioVision's Phospholipase C Activity Assay Kit is a direct assay to determine the PLC activity in various biological samples. The assay uses a specific PLC chromogenic substrate to detect PLC activity and the generated final product can be measured colorimetrically at OD 405 nm. The OD signal is proportional to the PLC activity. The assay is rapid, sensitive and is a convenient tool for detecting PLC activity. It can detect as low as 0.1 mU PLC activity under the assay conditions.
Figure: PLC activity detected in rat brain, kidney and muscle lysates. Assay was performed according to the kit protocol.
For more details on this assay kit, visit: https://www.biovision.com/phospholipase-c-activity-assay-kit-colorimetric.html