Glucosylceramidase Activity Assay Kit

MILPITAS, Calif. - June 12, 2019 - PRLog -- Lysosomal glucosylceramidase also  known  as acid  beta-glucosidase (E.C.3.2.1.45)  is a lysosomal  membrane  protein  that cleaves  the beta-glucosidic linkage of glycosylceramide (GC).The enzyme plays a central role in the degradation of complex lipids and the turnover of cellular membranes. Through  the  production  of  ceramides, it participates in the  PKC-activated salvage  pathway  of  ceramide  formation. Glucosylceramidase also plays a role  in cholesterol metabolism. It may catalyze the glucosylation of cholesterol through a transglucosylation reaction that transfers glucose from glucosylceramide  to cholesterol. Deficiency  of Glucosylceramidase results in the accumulation of GC in lysosomes. This leads to Gaucher disease, an inherited disorder that affects many organs and tissues.
BioVision's Glucosylceramidase Activity Assay Kit provides a simple, rapid way to monitor Glucosylceramidase activity in a wide variety of biological samples. In this kit, Glucosylceramidase cleaves a specific synthetic substrate and releases a fluorophore, which can be easily quantified (Ex/Em=360/445 nm). The  assay is specific,  sensitive  and  can  detect  as low  as 0.2  μU of glucosylceramidase activity  in  variety  of samples.

Figure: Measurement of Glucosylceramidasein MDA-MB-231 Cell Lysates (10 μg protein), 3T3 Cell Lysates (5μg protein) and Mouse Spleen Extracts (5 μg protein). All assays were performed following kit K2003 protocol.

For more information on this kit, visit: https://www.biovision.com/glucosylceramidase-activity-ass...