Two Research Articles Cite HemogloBind™ To Reduce Analytical Interferences
O'Connell, Grant C., et al. "Monocyte-lymphocyte cross-communication via soluble CD163 directly links innate immune system activation and adaptive immune system suppression following ischemic stroke (https://www.nature.com/
Laing, Richard W., et al. "The use of an acellular oxygen carrier in a human liver model of normothermic machine perfusion (https://www.ncbi.nlm.nih.gov/
In the first reference, the purpose of this study was to investigate CD163 as a possible effector of stroke-induced adaptive immune system suppression as this is a scavenger receptor expressed on innate immune cell populations. A soluble peptide with lympho-inhibitory properties can be shed from the plasma membrane via the metalloprotease ADAM17. The article states "Total hemocyte fractions were thawed, mixed with NP-40 lysis buffer (Thermo Fisher) at a 1 to 1 ratio, and depleted of hemoglobin via the HemogloBind hemoglobin removal kit (Biotech Support Group, Monmouth Junction, NJ)… ADAM17 activity was measured in 200 ug of total hemocyte lysate". The authors provide novel evidence that the innate immune system employs protective mechanisms aimed at mitigating the risk of post-stroke autoimmune complications, and that CD163 is a key mediator of this phenomenon.
In the second reference, the authors present the first experience of using an acellular hemoglobin-based oxygen carrier (HBOC) Hemopure in a human model of Normothermic machine perfusion of the liver (NMP-L). This is a novel technique that preserves liver grafts under near-physiological conditions while maintaining their normal metabolic activity. The article states "All perfusates underwent haemoglobin depletion using Hemoglobind (BioTech Support Group LLC, Monmouth Junction, NJ) as per the manufacturer's instructions, except using a 1:8 ratio to ensure removal of all free haemoglobin. Perfusate levels of 8-OH-dG were quantified using a competitive ELISA (Abcam) as per the manufacturers protocol" .The authors conclusion is that Hemopure can be used as an alternative oxygen carrier to packed red cells in NMP-L perfusion fluid.
"The large amount of hemoglobin in samples of this type presents a special challenge for quantitative analysis. These articles demonstrate yet again that HemogloBind™
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Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For this market, key products include: AlbuVoid™ and AlbuSorb™ for albumin depletion, Cleanascite™
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