AnaSpec Highlights Calcein AM with High Purity Grades

Aug. 12, 2011 - PRLog -- AnaSpec is pleased to provide Calcein AM at two purity grades, >90% or >95%, as determined by HPLC. AnaSpec also offers the SensoLyte® Calcein Cell Viability Assay Kit - a rapid and sensitive assay to detect viable cells. This kit provides ample reagents (dye and assay buffer) for 1000 assays in a 96-well plate format.

Calcein AM (Calcein acetoxymethyl ester), a non-fluorescent, cell permeable compound, converts to the strongly green fluorescent calcein when hydrolyzed by intracellular esterases in live cells.1-2 It is widely used for determining cell viability since the fluorescence intensity of calcein is proportional to the amount of live cells. Calcein is a photostable reagent and is well retained in the cytoplasm of live cells. Calcein is not influenced by intracellular pH. Its bright green fluorescence can be monitored at Ex/Em=494nm /520 nm.

Amongst other reagents, including BCECF, AM and Carboxy-fluorescein diacetate, Calcein AM is the most suitable indicator for staining viable cells due to its low cytotoxicity property. Calcein AM does not affect cell proliferation or viability.2-3 Calcein AM is used in cell invasion, adhesion, migration and other cell-based assays.
AnaSpec’s Calcein AM, a green fluorescent cell viability indicator, has excellent quality, and is cost-effective.

For more information, please visit  http://www.anaspec.com/products/promotions.asp?id=86&col=...

References
1. Braut-Boucher, F. et al. J. Immunol. Methods 178, 41 (1995).
2. Akeson, AL. et al. J. Immunol. Methods 163, 181 (1993).
3. Weston , SA. et al. J. Immunol. Methods 133, 87 (1990).

AnaSpec, EGT Group

AnaSpec, EGT Group is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and qPCRs.  AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery.  AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis.  AnaSpec is certified to ISO 9001:2008.

For more information visit www.anaspec.com

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AnaSpec, Inc. is a leading provider of integrated proteomics solutions™ for worldwide life science research. AnaSpec offers expertise in three primary technologies: peptides, detection reagents, and combinatorial chemistry.