ER Transcription Factor Activity Assay Kit (Colorimetric)

A 96-well plate based colorimetric assay to measure the activation of estrogen receptor (human, mouse and rat) in nuclear extracts or cell lysates.
MILPITAS, Calif. - Sept. 13, 2021 - PRLog -- Estrogens including estrone, estradiol, estriol, and estretrol belong to the family of organic compounds known as steroids. They are primarily synthesized in the ovaries, but also in the adrenal glands and adipose tissue. They are involved in regulating many physiological processes including reproduction, cardiovascular health, bone integrity, cholesterol mobilization, brain function etc.

Estrogen mediates its action by binding to the estrogen receptor (ER) in the nucleus. The receptors then dimerizes and bind to specific response elements known as estrogen response elements (EREs) in the promoters of the target genes.

BioVision's ER Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of ER in nuclear extracts or cell lysates. The kit offers easy, rapid, sensitive and non-radioactive way to detect the activation of transcription factors in samples. In this assay, double stranded DNA sequence containing the ERE is coated on the 96-well plate. Active ER in the cell lysate or the nuclear extract binds to the oligonucleotides on the plate.

After the addition of ER primary antibody that recognizes the ER-ERE comlex, a HRP-conjugated secondary antibody is added followed by the addition of TMB substrate and a color signal is developed, which is measured at 450 nm.

Figures: Transcription factor activity assay using nuclear extracts of MCF7 cells with or without H2O2 treatment (A, B). Assay was performed following the assay kit protocol.

Storage Conditions: Multiple Temperatures
USAGE: For Research Use Only! Not For Use in Humans.
For more information on this Assay Kit Cat#  K2105

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