NFkB RelA/p65 Transcription Factor Activity Assay Kit (Colorimetric)

Semi-quantitative measurement of activation of human NFkB p65 in nuclear extracts or cell lysates.
MILPITAS, Calif. - July 26, 2021 - PRLog -- Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) represents a family of inducible transcription factors, which regulates many biological processes including immune responses, cell growth, apoptosis etc. This family includes five structurally related members namely NF-κB1 (or p50), NF-κB2 (or p52), RelA (or p65), RelB and c-Rel, which are normally sequestered in the cytoplasm by a family of inhibitory proteins and are inactive.

Upon stimulation, NF-κB proteins form homo- or heterodimers and are transported into the nucleus where they bind to specific DNA sequences of targeted genes and activate transcription. Traditionally, western blot is used to detect the expression of NFkB and electrophoretic mobility shift assay or reporter assays are used to measure the NFkB activity respectively. But some of these methods are time consuming, laborious, and require radioactivity. BioVision's NFkB RelA/p65 Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of human NFkB p65 in nuclear extracts or cell lysates.

The kit offers easy, rapid, sensitive and non-radioactive way to detect the activation of transcription factors in samples. In this assay, double stranded DNA sequence containing the NFkB p65 consensus binding site is coated on the 96-well plate. Active NFkB p65 in the cell lysate or the nuclear extract binds to the oligonucleotides on the plate. After the addition of RelA/p65 primary antibody that recognizes the NFkB p65-oligonucleotide complex, a HRP-conjugated secondary antibody is added followed by the addition of TMB substrate and a color signal is developed, which is measured at 450 nm.

Storage Conditions: Multiple Temperatures
USAGE: For Research Use Only! Not For Use in Humans
For more information on this Assay Kit Cat# K2093

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