Cyclic Adenosine Monophosphate (cAMP) Assay Kit (Fluorometric)

MILPITAS, Calif. - June 9, 2021 - PRLog -- Cyclic Adenosine Monophosphate (cyclic AMP or cAMP) is a vital second messenger found in eukaryotic and prokaryotic cells. It regulates many biological pathways such as glucose and lipid metabolism, cell growth and differentiation, gene transcription, protein expression etc. Additionally, deregulation of cAMP signaling has been shown in cancer development and progression.

The main effectors of cAMP include protein kinase A (PKA), guanine-nucleotide-exchange factor and cyclic nucleotide gated ion channels. PKA is a tetrameric holoenzyme consisting of two catalytic subunits and a regulatory subunit dimer and its activity is dependent on the cellular levels of cAMP. Upon elevation of intracellular cAMP levels, cAMP binds to the regulatory subunits of PKA thereby causing a conformational change and releasing the catalytic subunit, which can then phosphorylate the substrate proteins and increase the transcription of target genes.

BioVision's cAMP Assay Kit provides a rapid, sensitive method to detect cAMP in various sample types. In this assay, cAMP is converted to AMP in the presence of a phosphodiesterase. AMP then goes through several enzymatic reactions to produce an intermediate, which reacts with a probe to generate a fluorescent signal measured at Ex/Em = 535/587 nm. The fluorescent signal is proportional to the amount of cAMP present in the sample. The assay is simple, easy to use, quick, sensitive, and is high-through adaptable. It can measure lower than 0.2 µM cAMP levels in various samples.

Figures: (a) cAMP Standard Curve. (b) Measurement of cAMP in Jurkat cells without or with 100 µM NHK477 treatment. (c) Measurement of cAMP in HeLa cells without or with 100 µM NHK477 treatment. Assays were performed following the protocol.

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