Elastin Assay Kit (Colorimetric)

A quantitative dye-binding based method for the analysis of elastin levels in tissues and cultured cells.
 
MILPITAS, Calif. - March 3, 2021 - PRLog -- Elastin is a key protein of the extracellular matrix that provides resilience and elasticity to tissues and organs. It is highly elastic and is primarily present in extensible tissues including arteries, lung, ligaments and skin to resume their shape after stretching or contracting. Because of its critical role in normal development and function of vital organs, either an impairment of elastin synthesis or proteolytic degradation of the insoluble elastin fibers results in major clinical pathologies. Mutations in the Elastin gene may lead to diseases such as Williams-Beuren syndrome, Cutis laxa and Supravalvular aortic stenosis.

Additionally, a number of skin diseases have been associated with abnormalities of elastin and marked fragmentation of elastin is observed in pulmonary emphysema.

BioVision's Elastin Assay Kit is a quantitative dye-binding based method for the analysis of elastin levels in tissues and cultured cells. Due to its unique chemical composition and highly cross-linked nature, elastin is stable. But in this kit, elastin is denatured and solubilized under high heat and extreme pH conditions unlike other proteins. The isolated elastin then reacts with a dye to form a colored product, which is measured by absorbance at 513 nm. The colorimetric signal is directly proportional to the amount of elastin in samples. The assay is easy to perform, reliable and selective. It has a linear range of 5 μg-100 μg elastin per well.

Figures: (a). Elastin Standard Curve. (b). Estimation of purified elastin and collagen I (in 0.25M Oxalic Acid and 100 ºC for 1 hour). (c) Estimation of elastin content in rat heart (2.58 mg wet tissue/1 ml 0.25M Oxalic Acid), rat liver (3.55 mg wet tissue/1ml 0.25M Oxalic Acid) and rat lung (2.88 mg wet tissue/1 ml 0.25M Oxalic Acid) samples. (d). Estimation of elastin content in CHO cells (106 cells/500 μl 0.25M Oxalic Acid) and HEK293 cells (106 cells/1 ml 0.25M Oxalic Acid). Data are mean ± SEM of 3 replicates, assayed according to the kit.

For more information on this Assay kit Cat# K2077, Visit: https://bit.ly/30bpCzy

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