Mammalian RNA Isolation Assay Kit

MILPITAS, Calif. - Feb. 19, 2021 - PRLog -- BioVision's Mammalian RNA Isolation Kit allows the quick and simple extraction of RNA from mammalian cells and tissues. Samples are first lysed and homogenized in a specially designed lysis buffer, which inhibits the activity of RNases. After the addition of ethanol, the sample is applied to the RNA column to bind RNA. After 2 wash steps, purified RNA is eluted with RNase-free water. Purified RNA can be used for downstream applications such as RT-PCR, qPCR, etc.

Additionally, this kit can be used for clean-up of RNA and RNA enzymatic reactions. The kit enriches for RNA > 200 bp as smaller RNAs do not bind to the column efficiently.

Figure 1: RNA was purified from 5 x106 (Lanes 1 & 3) and 1x107 (Lanes 2 & 4) Jurkat cells stored in RNAkeepTM Solution (BioVision Cat# M1241) using Mammalian RNA Isolation Kit (BioVision Cat# K2065). 10 μl RNA was loaded per lane and analyzed on a 1% agarose gel. RM is Riboruler marker.

For more information on this Assay kit Cat# K2065, Visit: https://bit.ly/3qCW3m2