Malaria research article cites use of HemogloBind™ in reticulocyte study
By: Biotech Support Group
The citation is:
Hojo-Souza NS, de Azevedo PO, de Castro JT, Teixeira-Carvalho A, Lieberman J, et al. (2020) Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage. PLOS Pathogens 16(9): e1008840. https://doi.org/
The authors describe how P. vivax-infected Retics (iRetics) express human leukocyte antigen class I (HLA-I), which are recognized by CD8+ T cells and killed by granulysin (GNLY) and granzymes. However, how Plasmodium infection induces MHC-I expression on Retics is unknown. In addition, whether GNLY helps control Plasmodium infection in vivo has not been studied. To remove interferences associated with Hemoglobin, the article states, "For western blot analysis, erythroblasts pellets were resuspended in RIPA Buffer (Sigma)…. The Retics were treated with HemogloBind (Biotech Support Group) …". The research results indicate that in addition to previously described mechanisms, IFN-γ promotes host resistance to the Retic-tropic P. yoelii 17XNL strain by promoting MHC-I expression on iRetics that become targets for CD8+ cytotoxic T lymphocytes and GNLY.
"I am pleased to see another use for HemogloBindTM in malaria research of reticulocytes. With the efficiency of Hemoglobin removal that our products provide, investigators can now study the many aspects of red cells as they relate to disease. Finally, we can provide a simple method to remove Hemoglobin interference, as this can often be a problem with immunoassay and Western blot analysis. Furthermore, this article demonstrates compatibility with the commonly used RIPA lysis buffer.", states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
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