Choline Oxidase Activity Assay Kit (Fluorometric)

MILPITAS, Calif. - Sept. 15, 2020 - PRLog -- Choline Oxidase (E.C. 1.1.3.17) belongs to the oxidoreductase family and is present in plants, bacteria, fungi and mammals. It is a catabolic enzyme that catalyzes the chemical reaction of choline and oxygen into betaine glycine and hydrogen peroxide with betaine aldehyde as an intermediate. The enzyme participates in glycine, serine, and threonine metabolism and is commonly used as a tool for the enzymatic determination of acetylcholinesterase, phospholipase D, phosphatidylcholine-specific phospholipase C, sphingomyelinase etc.

Betaine glycine is a well known osmoprotectant and can help plants and bacteria to survive dry, cold and salt stresses. As a result, choline oxidase has been engineered genetically to produce transgenic plants such as tomato, rice and tobacco with increased stress tolerance and productivity. Additionally, accumulation of betaine glycine has been observed in bacteria such as Staphylocccus aureus, Pseudomonas aeruginosa and Listeria monocytogenes thereby suggesting that drugs capable of inhibiting choline oxidase activity will have potential anti-bacterial activity.

BioVison's Choline Oxidase Activity Assay Kit is the first, commercially available kit to determine the activity of choline oxidase in samples. In this assay, choline oxidase oxidizes choline to generate hydrogen peroxide, which then reacts with the probe to generate a strong fluorescent signal measured at Ex/Em = 535/587 nm. The fluorescent signal is directly proportional to the choline oxidase activity in the samples. The assay is simple, fast, reproducible and is high throughput adaptable. The kit can detect as low as 0.2 mU of choline oxidase activity under assay conditions.

Figures. A). H2O2 Standard Curve. B). Reaction curve of Choline Oxidase activity (Positive Control vs Background). C). Reaction curve of Choline Oxidase in bacterial cell lysates before and after spiking with 0.2 mU of Choline Oxidase. Assay was performed according to kit K2052 assay protocol.

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