- June 17, 2020
-- N-Acetylcysteine (NAC) is a precursor of L-cysteine and glutathione biosynthesis. It is a powerful antioxidant and a scavenger of free radicals. It has been commonly used for treating cough, flu, dry eye, lung conditions, paracetamol intoxication etc. Additionally, recent investigations have explored the beneficial effects of NAC in type 2 diabetes and psychiatric disorders. NAC is available as over the counter in tablet, solution as well as intravenous and inhaled preparations. Common side effects of NAC treatment are generally mild and typically resolve on their own once the treatment is stopped. BioVision's N-Acetylcysteine Assay Kit is a simple and sensitive method for the quantitation of NAC in biological samples. The assay is based on the deacetylation reaction of NAC to generate cysteine. Cysteine is then catalyzed in subsequent reactions thereby producing an intermediate product, which reacts with a fluorogenic probe to form a stable fluorophore measured at Ex/Em = 368/460 nm. The assay is specific with no interference from other thiol-based amino acids.
Determining the concentration of N-Acetylcysteine in biological samples and pharmaceuticals. Sample Type: Biological samples (serum, plasma, etc.).
Figures: (a) NAC Standard Curve. (b) Estimation of NAC in single-donor human serum and plasma (5 μl), spiked with 5 nmol of NAC Standard (equivalent to 20 μM free NAC). Total NAC concentrations for serum and plasma were 4.8 + 0.3 μM and 0.7 + 0.0 μM with average spiked recoveries of 92.0% and 93.5%, respectively. The assay was performed according to the kit assay protocol.
For more information on this Assay Kit, visit: https://www.biovision.com/n-acetylcysteine-assay-kit-fluorometric.html