MILPITAS, Calif. -
Feb. 25, 2020 -
PRLog -- Laccases (EC 1.10.3.2), also known as p-benzenediol:
oxygen oxidoreductases are a class of copper-containing enzymes found in bacteria, insects, plants and fungi. They catalyze the oxidation of aromatic compounds using molecular oxygen as the terminal electron acceptor and their molecular mass ranges from 60-100 kDa. Laccases play a key role in lignin synthesis and degradation and are utilized in several biotechnological applications such as degradation of dyes, chemical wastes, xenobiotics and other phenolic compounds. Additionally, Laccases are used in food industry for producing cost-effective, healthy foods. Furthermore, they are also being investigated in the construction of Laccase biosensors because of their high redox potential. BioVision's Laccase Activity Assay Kit is a simple, one step, plate based assay to measure Laccase activity in various samples. In this assay, samples containing Laccase will oxidize the substrate to generate a colored product with a strong absorbance at 420 nm. The kit is rapid, easy to use and high-throughput adaptable. It can measure Laccase activity as low as 20 µU in samples.
Figure: (a). Enzyme kinetics using mushroom lysate (5 µg protein per well) and potato lysate (0.62 µg protein per well) (b). Laccase specific activity in potato and mushroom lysate. Experiments were conducted according to kit K2038 protocol.
For more information on this kit, visit:
https://www.biovision.com/laccase-activity-assay-kit-colorimetric.html
