MILPITAS, Calif. -
Aug. 26, 2019 -
PRLog -- L-Citrulline is a non-proteogenic, semi-essential amino acid. Citrulline is formed either by ornithine carbamoyltransferase or as a byproduct of the Nitric oxide synthase (NOS) activity. Citrulline generated in the NOS reaction can be recycled to arginine by the two enzymes argininosuccinate synthetase (ASS) and argininosuccinate lyase (ASL) acting in the Urea Cycle. Citrulline concentrations have been shown to be elevated in patients with mutated ASS or ASL genes. Watermelon is one of the richest sources of Citrulline. Additionally, Citrulline has been advertised as sports nutrition supplement due to its involvement in Nitric Oxide (NO) synthesis, which helps with vasodilation. BioVision's Citrulline Assay Kit provides a rapid, specific, and easy method for the measurement of total Citrulline concentrations in a wide variety of samples. In this enzymatic assay, Citrulline is converted into a series of intermediates, which further reacts with a probe producing a stable fluorescent signal (Ex/Em = 535/587 nm). The kit is simple, easy to perform, sensitive and is highthroughput adaptable. It can detect as low as 2 µM Citrulline in biological samples.
Figure: Estimations of Citrulline in 2 human serum samples (10 and 40 µl in each well respectively)
and watermelon juice (4 µl of 100X dilution). Citrulline concentrations were 33.50 µM and 9.11 µM in human serum respectively and 10.81 mM in Watermelon Juice. Assays were performed following the kit K2002 protocol.
For more information on this kit, visit:
https://www.biovision.com/citrulline-fluorometric-assay-kit.html
