EZCell™ Intracellular Zinc Detection Kit (Cell-Based) (K525-50)

MILPITAS, Calif. - Feb. 23, 2018 - PRLog -- Zinc (Zn, Atomic Number: 30) is an essential mineral of great biological significance. It is a metal with characteristics similar to Magnesium due to its size and +2 oxidation state. It is the second most abundant transition metal in living organisms after iron. Zn²⁺ binding proteins account for nearly 50% of the transcription regulatory proteins in the human genome and require zinc as an essential cofactor in the regulation of gene expression. Numerous enzymes also employ Zn²⁺ ions in their active sites. Free Zn²⁺ is released from metalloprotein complexes during oxidative stress with the remainder being bound to proteins or nucleic acids. Other examples of biological roles of Zinc include signal transduction, regulation of apoptosis, neurotransmission, synaptic plasticity and prostate gland function. In humans, Zinc deficiency is associated with many chronic diseases such as renal disease, sickle cell anemia, cirrhosis, some cancers and deficiencies in metabolism.

BioVision has developed the EZCell™ Intracellular Zinc Staining Kit (cell-based) which contains a selective and membrane permeable fluorescent probe for detection of intracellular Zinc. We also include a Positive Control that elevates Zinc staining, and cell-permeable Zinc Chelator that reduces fluorescence induced by Positive Control. This easy-to-use non-radioactive assay allows studies of the regulation of Zinc at the cellular level by Fluorescence Microscopy and Flow Cytometry in cultured cells.

Key Features:

• Simple procedure; takes ~ 2 hours

• Fast and convenient

• The assay is sensitive, stable, specific and high-throughput adaptable

• Applications- the kit can visualize intracellular content in suspension and/or adherent cells


Figure: Zinc Staining in Jurkat and HeLa cells. 1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control, or Positive Control plus Zinc Chelator for 30 minutes followed by Zinc Staining according to kit's protocol. Histograms from flow analysis of untreated cells stained with Zinc Probe (Green Line) and cells treated with Positive Control (Pink Line) show increased signal in cells treated with Positive control reagent compared to untreated cells

For detailed information on this product please visit: https://www.biovision.com/ezcelltm-intracellular-zinc-sta...