Nov. 30, 2011 -
PRLog -- WILMINGTON, Del. (November 30 2011) – Thermo Fisher Scientific Inc., the world leader in serving science, today announced that the University of California (UC), San Diego, Division of Nephrology/Hypertension has implemented a Thermo Scientific NanoDrop 3300 Fluorospectrometer as the fluorescent detection system for mouse glomerular filtration rate (GFR) determination. The Thermo Scientific NanoDrop 3300 Fluorospectrometer offers a reliable micro-volume fluorescent platform for repeated determination of GFR in conscious mice to assess their renal function without surgical procedures. The instrument allows scientists to perform these measurements simply, quickly and cost-effectively, making the instrument a valuable tool for GFR research applications. A new application note entitled ‘Measuring Mouse GFR by Fluorescein isothiocyanate (FITC)-Inulin using the Thermo Scientific NanoDrop 3300 Fluorospectrometer’
, is available for download.
UC San Diego’s Division of Nephrology/Hypertension considers GFR measurement to be the most reliable method in renal function assessment and as such the technique is utilized by the team to determine GFR under physiological and pathophysiological conditions in mice. In the past, the division determined GFR by measuring radioactively labeled inulin in blood plasma and urine in anesthetized animals. However, anesthesia has multiple effects on the physiology of the mammalian body and repetitive measurements are difficult to perform. As this limitation significantly impacted the study designs, the division sought an alternative method.
UC San Diego’s Division of Nephrology/Hypertension has implemented the Thermo Scientific NanoDrop 3300 fluorospectrometer to enable the measurement of GFR using FITC-inulin. This protocol retains the sensitivity of the traditional assay by using fluorescence detection, while the instrument’s microvolume technology has enabled researchers to reduce the volumes of blood required to
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