Biases in α-synuclein immuno-quantitation

BORDEAUX, France - April 7, 2024 - PRLog -- Biases in α-synuclein immuno-quantitation: a core problem for basic and ancillary studies of Parkinson's disease and multiple system atrophy

By: Florent Laferrière, Ludivine Sabatier, Stéphane Claverol, Francesca De Giorgi and François Ichas

In this study, we identified several pitfalls in the analysis of amyloid α-synuclein (α-syn) from brain samples of Parkinson's disease and multiple system atrophy. Notably, i) the unsuitability of SDS-PAGE to resolve these assemblies, ii) the specific loss of physiological forms of α-syn during immunoblotting procedures, and iii) the inability of a commonly used antibody to detect α-syn amyloids. The latter issues distort the quantitation of amyloid / total α-syn in biological samples, and represent a significant bias in the evaluation of its structural or functional properties in the pathology. We developed user-friendly immunoblotting and ELISA procedures to circumvent these pitfalls.

Link to the article in published in  "Translational Neurodegeneration" :
https://doi.org/10.1186/s40035-024-00408-x