Research Article Cites Cleanascite™ In Lipid Factor Determination of Immune Response in Plants

By: Biotech Support Group
 
MONMOUTH JUNCTION, N.J. - Oct. 10, 2020 - PRLog -- Biotech Support Group reports on a book chapter describing the simplicity and efficiency of their lipid clearance sample preparation technology for the preparation and storage of monoclonal antibodies. The citation is:

Stahl, Elia, et al. "Phosphatidylcholines from Pieris brassicae eggs activate an immune response in Arabidopsis (https://elifesciences.org/articles/60293)." eLife 9 (2020): e60293.

Recognition of conserved microbial molecules activates immune responses in plants, a process termed pattern-triggered immunity (PTI). Similarly, insect eggs trigger defenses that impede egg development or attract predators, but information on the nature of egg-associated elicitors is scarce. The authors performed an unbiased bioactivity-guided fractionation of eggs of the butterfly Pieris brassicae to determine the nature of egg-associated molecular patterns (EAMPs) that induce immune responses in Arabidopsis.

Preliminary tests indicated that egg-derived defense eliciting compounds are of lipidic nature. To confirm this, the authors stated "we used Cleanascite solid-phase aqueous reagent to selectively adsorb lipids from EE (Egg Extract). Application of the lipid-containing phase to …Arabidopsis reporter lines triggered strong and localized GUS staining, similar to EE treatment. In contrast, the supernatant containing proteins and other non-lipidic molecules was not active, indicating that defense gene-inducing molecules were restricted to the lipid phase (Figure 1A)". This helped the research in the identification of phosphatidylcholines (PCs) as egg-associated molecular patterns (EAMPs), and illustrated the acute ability of plants to detect conserved immunogenic patterns from their enemies, even from seemingly passive structures such as eggs.

"Its rewarding to see another reference showing that Cleanascite™ helped to identify previously unknown lipid factors which produced an immune response in plants. As Cleanascite™ is an aqueous suspension product without harmful solvents, one can use it to challenge cells for responsiveness. This ultimately helps researchers decide whether or not lipids, or factors interacting with lipids, impart characteristic changes in cells." states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

To download whitepaper entitled "Cleanascite™ - Lipid Removal and Cell Response Applications", visit:

http://www.biotechsupportgroup.com/v/vspfiles/templates/257/pdf/CleanasciteCellResponseReferenceApplications121218.pdf

For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For more information, go to http://www.biotechsupportgroup.com.

For Business Development, contact: Matthew Kuruc 732-274-2866, mkuruc@biotechsupportgroup.com (mailto:sales@biotechsupportgroup.com)

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Tags:Immune Response
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