Research Article Cites Cleanascite™ In Lipid Factor Determination of Immune Response in Plants
By: Biotech Support Group
Stahl, Elia, et al. "Phosphatidylcholines from Pieris brassicae eggs activate an immune response in Arabidopsis (https://elifesciences.org/
Recognition of conserved microbial molecules activates immune responses in plants, a process termed pattern-triggered immunity (PTI). Similarly, insect eggs trigger defenses that impede egg development or attract predators, but information on the nature of egg-associated elicitors is scarce. The authors performed an unbiased bioactivity-
Preliminary tests indicated that egg-derived defense eliciting compounds are of lipidic nature. To confirm this, the authors stated "we used Cleanascite solid-phase aqueous reagent to selectively adsorb lipids from EE (Egg Extract). Application of the lipid-containing phase to …Arabidopsis reporter lines triggered strong and localized GUS staining, similar to EE treatment. In contrast, the supernatant containing proteins and other non-lipidic molecules was not active, indicating that defense gene-inducing molecules were restricted to the lipid phase (Figure 1A)". This helped the research in the identification of phosphatidylcholines (PCs) as egg-associated molecular patterns (EAMPs), and illustrated the acute ability of plants to detect conserved immunogenic patterns from their enemies, even from seemingly passive structures such as eggs.
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