Insightful Advice for Avoiding Clonal Deletions

Nov. 14, 2012 - PRLog -- Integrated DNA Technologies (IDT) (http://eu.idtdna.com/site), the world leader in oligonucleotide synthesis, offers valuable advice in the October edition of DECODED to help avoid deletions that sometimes occur during cloning. For reasons that are not well defined, scientists who use oligonucleotide primers for PCR amplification prior to cloning occasionally obtain sequence deletions across multiple clones, often affecting the same, singular base. While site-directed mutagenesis can resolve cloning deletions (see the free IDT Mutagenesis Application Guide for help with this approach), optimizing the process to avoid deletions is often more desirable, saving time and cost. The cloning tips from IDT to increase the odds of generating correct clones are featured in the latest edition of the DECODED newsletter.

Read the article (http://eu.idtdna.com/pages/decoded/decoded-articles/pipet-tips/decoded/2012/09/21/deletions-during-cloning) in the October 2012 edition of DECODED (http://eu.idtdna.com/pages/decoded/decoded-2-4). For more information on IDT products and services please visit www.idtdna.com. For real-time news and updates, follow IDT on Twitter @idtdna or find us on Facebook.

Editors’ notes

About IDT

Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the world, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; Leuven, Belgium; and Singapore. For more information, visit www.idtdna.com.

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