Albumin depletion for secretome sample preparation via AlbuVoid™ for cellular modeling systems
Researchers used AlbuVoid™ for serum albumin depletion for secretome sample preparation. Albumin was depleted from cell culture in serum containing medium using AlbuVoid™ to isolate and identify low abundance proteins.
The patient cites AlbuVoid™ from Biotech Support Group
“In one embodiment, the cells can be cultured in serum containing medium: The volume of the medium can be reduced using 3k MWCO Vivaspin columns (GE Healthcare Life Sciences), then can be reconstituted withlxPBS (Invitrogen)
Characteristics of AlbuVoid™
Albumin voids in flow-through >95%, with <30 minute bind/wash/elute protocol
Low abundance enrichment equivalent or better than hexa-peptides or antibodies
Disposable, cost-effective, no column regeneration or cross-contamination
Mild elution maintains tertiary structure and simple transfer to secondary analysis
The eluted fractions retain their enzymatic and biological activity
Removes albumin from many species including human, mouse, sheep, bovine, goat, rat, calf, cell culture. The eluted fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.
For more information about AlbuVoid™, click:
AlbuVoid™ - Albumin Depletion Kit from Serum or Plasma
About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuVoid™
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Serum Profiling Making Mark on Predictive Medicine
Vicki Glaser.Genetic Engineering & Biotechnology News. 2011;31(7):1-
Narain, Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas. "INTERROGATORY CELL-BASED ASSAYS AND USES THEREOF." U.S. Patent No. 20,120,258,874. 11 Oct. 2012.