White Paper Explores Effective Protein Purification

A new METTLER TOLEDO white paper entitled "High Throughput FPLC with Packed Resin Tip Technology" describes how novel PureSpeed packed resin tips promote high-throughput protein purification
 
 
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Tags:
* Effective Protein Purification
* Greater Efficacy
* Bidirectional Flow

Industry:
* Biotech

Location:
* Greifensee - Zurich - Switzerland

Nov. 6, 2012 - PRLog -- A new METTLER TOLEDO white paper entitled "High Throughput FPLC with Packed Resin Tip Technology" describes how novel PureSpeed packed resin tips promote high-throughput protein purification, offering greater efficacy, time and cost-savings than can be typically gained using traditional spin and gravity columns.

Rainin Instrument, a METTLER TOLEDO subsidiary, has published a new white paper entitled "High Throughput FPLC with Packed Resin Tip Technology." This addition to METTLER TOLEDO's thought-leadership library helps shed light on how novel PureSpeed resin tips promote easier, faster and more cost-effective protein and antibody purification than can be achieved using traditional gravity/spin columns.

Using conventional spin columns, protein purification requires a relatively large amount of resin for acceptable performance. However, experiments detailed in the white paper indicate that the higher resin volumes required in spin columns are not the key to optimizing workflow.

PureSpeed's bidirectional flow was shown to load PureSpeed's relatively small resin bed with the most protein possible, providing superior final concentrations and purity when compared to the spin column procedure. Protein capture was shown to be more repeatable as well, even at significantly reduced elution volumes.

The improved concentrations that PureSpeed enables help provide samples robust enough for assaying or downstream processing. Generally, this saves time by eliminating the need to concentrate recovered protein in a separate step.

The paper also offers guidelines for using PureSpeed tip, body and bed sizes to gain maximum protein/antibody processing.

For more information, please visit http://us.mt.com/us/en/home/supportive_content/White_Pape...

About Mettler Toledo:
METTLER TOLEDO specializes in the area of precision instruments for professional use. METTLER TOLEDO laboratory instruments are used in research, scientific, drug discovery, and quality control labs, amongst many others in the pharmaceutical, chemical, food and cosmetics industries. METTLER TOLEDO comprehensive industrial solutions cover the various steps in a host of manufacturing processes at many of the same customers that laboratory serves. Solutions range from receiving raw materials through various manufacturing processes, in-line process control and end-of-line packaging control, to logistics and shipping. Increasingly, these solutions are fully integrated into the customer's IT environment, helping automate their workflows. In food retail, our offering for fresh food management ranges from receiving and pre-packaging, to in-store solutions for self-service departments, deli counters, and checkout terminals.
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Tags:Effective Protein Purification, Greater Efficacy, Bidirectional Flow
Industry:Biotech
Location:Greifensee - Zurich - Switzerland
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