tebu-bio launches ProtOptimiX® – protein expression optimisation

Constantly seeking to enrich their range of laboratory services, tebu-bio is now offering Life Sciences researchers a standardized protein expression optimisation service in E. coli.
By: Sara Pierre
 
 
Typical results following ProtOptimix optimization
Typical results following ProtOptimix optimization
 
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Tags:
Tebu-bio
Protoptimix
E Coli
Protein
Expression
Optimization

Industrys:
Biotech
Research
Science

Location:
Le Perray-en-Yvelines - Ile de France - France

July 29, 2010 - PRLog -- tebu-bio laboratories, who are celebrating their 10th anniversary this year, have acquired solid experience in E. coli protein production, with their large, well equipped facilities allowing for multiple projects to be handled simultaneously.

This solid background has led to the development of a standard optimisation service, simply requiring the customer to provide their gene of interest cloned in an expression vector or the transformed bacteria. Expression of the protein is then tested, with results generally provided in approximately two weeks following commencement of the project. Four important parameters are monitored (temperature, induction time, inducer concentration & induction duration) and full quality control is performed by SDS-PAGE with Coomassie staining and Western Blot.

“Our experience shows that for 8 projects out of 10, protein expression in E. coli can be optimised, or even just simply be made possible by a multi-parametric approach”, says Olivier Roumanie (Project Manager, tebu-bio laboratories). "However, a restricting factor is that this Design of Experiment is often long and cumbersome, requiring permanent access to several dedicated incubators". Gilles Cestelli (Brand Manager, tebu-bio) adds  “We are very pleased to be able to make this service available to our customers, allowing them to focus on their research while we take care of their project” .

This service complements the range of services available from tebu-bio, such as the cell line engineering services SilenciX® & ExpressiX® (stably silenced cell lines & custom services to stably over-express a gene of interest – http://www.tebu-bio.com/to/CellLineServices).

To find out more about ProtOptimix®, visit http://www.tebu-bio.com/to/ProtOptimiX. Full information about other services by tebu-bio Laboratories can also be found at www.tebu-bio.com

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tebu-bio is an expert in the supply & distribution of Life Sciences Lab Services & Reagents throughout Europe. Their own laboratories also provide R&D contract services. www.tebu-bio.com offers access the most innovative range of over 730 000 references.
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