Innovative Method for 3D Quantitative Phase Imaging

LOS ANGELES - July 25, 2024 - PRLog -- A recent study from the University of California, Los Angeles, introduces a cutting-edge approach to 3D Quantitative Phase Imaging using a wavelength-multiplexed diffractive optical processor. Quantitative Phase Imaging (QPI) is a powerful technique that reveals variations in optical path length caused by weakly scattering samples, enabling the generation of high-contrast images of transparent specimens. Traditional 3D QPI methods, while effective, are limited by the need for multiple illumination angles and extensive digital post-processing for 3D image reconstruction, which can be time-consuming and computationally intensive.

In this innovative study, the research team developed a wavelength-multiplexed diffractive optical processor capable of all-optically transforming phase distributions of multiple 2D objects at various axial positions into intensity patterns, each encoded at a unique wavelength channel. This design (see Figure) allows for the capture of quantitative phase images of input objects located at different axial planes using an intensity-only image sensor, eliminating the need for digital phase recovery algorithms.

"We are excited about the potential of this new approach for biomedical imaging and sensing," said Aydogan Ozcan, lead researcher and Chancellor's Professor at UCLA. "Our wavelength-multiplexed diffractive optical processor offers a novel solution for high-resolution, label-free imaging of transparent specimens, which could greatly benefit biomedical microscopy, sensing and diagnostics applications."

The innovative multiplane QPI design incorporates wavelength multiplexing and passive diffractive optical elements that are collectively optimized using deep learning. By performing phase-to-intensity transformations that are spectrally multiplexed, this design enables rapid quantitative phase imaging of specimens across multiple axial planes. This system's compactness and all-optical phase recovery capability make it a competitive analog alternative to traditional digital QPI methods. A proof-of-concept experiment validated the approach, showcasing successful imaging of distinct phase objects at different axial positions in the terahertz spectrum. The scalable nature of the design also allows adaptation to different parts of the electromagnetic spectrum, including the visible and IR bands, using appropriate nano-fabrication methods, paving the way for new phase imaging solutions integrated with focal plane arrays or image sensor arrays for efficient on-chip imaging and sensing devices.

This research has significant implications for various fields, including biomedical imaging, sensing, materials science, and environmental analysis. By providing a faster, more efficient method for 3D QPI, this technology can enhance the diagnosis and study of diseases, the characterization of materials, and the monitoring of environmental samples, among other applications.

Original paper: https://doi.org/10.1117/1.AP.6.5.056003