Enrichment of DNA From Protein Samples Using ProCipitate™

Aug. 19, 2012 - PRLog -- MONMOUTH JUNCTION, NJ.–In order to perform sequencing, polymerase chain reaction (PCR), microarrays and similar techniques for molecular biology studies, researchers routinely implement DNA extraction or enrichment techniques to separate DNA from protein samples. Traditionally DNA from biological samples such as blood, human, animal,plant tissues, bacteria and cell cultures is separated from DNA by a combination of proteinases and organic solvents, such as phenol. Phenol extraction method for deproteinization of nucleic acid solutions is toxic, requires redistillation before use and accumulates phenolic oxidation products (quinones, diacids, etc.) during storage which is purified using dialysis. ProCipitate™ is non-hazardous and can replace phenol/chloroform with the additional benefits of solid-phase suspensions: adaptability to filtration and automation. It is routinely used for plasmids, cosmids, BACs, and genomic DNA, as well as RNA. ProCipitate™ can also be used to remove Proteinase K and other enzymes. ProCipitate™ provides high quality DNA suitable for automated sequencing, southern blotting, and restriction digestion. ProCipitate™ binds proteins and DNA/RNA remains unreacted.

Preparation of large quantity and high quality genomic DNA requires gathering cells containing DNA, releasing DNA from collected cells, separation of DNA from proteins and cellular contaminants and isolation of concentrated DNA for use in genetic and genomic analyses techniques such as genetic mapping, TILLING (Targeting Induced Local Lesion IN Genome), and next-generation sequencing. ProCipitate™ is high throughput, high yield, and cost efficient for small or large sample genomic DNA enrichment. It is compatible with high throughput genomic DNA isolation and produces high quality, improved yield of DNA over alternative bind and elute systems. ProCipitate™ is available as a suspension reagent and in ProPrep™ kits for specific applications and high-throughput 96 well filter formats.

For more information click: http://www.biotechsupportgroup.com/node/114

About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include:  AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, and ProCipitate™ & ProPrep™ for nucleic acid isolation. Currently, Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a proteomics platform used in functional profiling for proteomic analysis and a separations method for generating sub-proteomes used in biomarker and functional proteomic prospecting. For more information, go to: www.biotechsupportgroup.com.

CONTACT:
Dr.Swapan Roy
Biotech Support Group            
1 Deer Park Drive, Suite M,
Monmouth Junction, NJ 08852, USA              
732-274-2866                    
sales@biotechsupportgroup.com

ProCipitate™ References:

Kozekov ID; Turesky RJ; Alas GR; Harris CM; Harris TM; Rizzo CJ. Formation of Deoxyguanosine Cross-Links from Calf Thymus DNA Treated with Acrolein and 4-Hydroxy-2-nonenal. Chemical Research in Toxicology.2010;23(11):1701-1713

Stephanie M Cohen, Terrence S Furey, Norman A Doggett, and David G Kaufman. Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts BMC Genomics.2006;7: 301

Krupey, J., et al, 100,000+ PCRs Possible from 10 ml Blood, poster Biotechniques Symposium, 2003.

Quiniou SM; Katagiri T; Miller NW; Wilson M; Wolters WR; Waldbieser GC Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus. Genetics, selection, evolution : GSE. 2003;35(6):673-83

J M Kelley; C E Field; M B Craven; D Bocskai; U J Kim; S D Rounsley; M D Adams. High Throughput Direct End Sequencing of BAC Clones. Nucleic Acids Research.1999.15;27(6):1539-1546

DNA Extraction Genetic Diversity as an Indicator of Ecosystem Condition and Sustainability for Regional Assessments of Stream Condition in the Eastern United States

David C. Bruce; Mark O. Mundt; Kim K. McMurry; Linda J. Meincke; Donna L. Robinson; Norman A. Doggett; Larry L. Deaven. BAC Library End Sequencing in Support of Whole Genome Assemblies. DOE Joint Genome Institute and Center for Human Genome Studies, Los Alamos National Laboratory

G.M. Huang; K. Wang; C.L. Kuo; B. Paeper; L. Hood A High-Throughput Plasmid DNA Preparation Method Analytical Biochemtry.1994;15;223(1):35-8

Robert R. Klein, Daryl T. Morishige, Patricia E. Klein, Jianmin Dong; John E. Mullet. High Throughput BAC DNA Isolation for Physical Map Construction of Sorghum. Plant Molecular Biology Reporter.1988;16(4):651-364