The citation is:
Barasa, Benjamin, and Monique Slijper. "Challenges for red blood cell biomarker discovery through proteomics."
In brief, this review describes the many challenges to generate in-depth RBC proteome analysis, such as to obtain pure red blood cells, and to acquire an in-depth proteome, despite the dynamic range problem due to a few highly over-represented RBC proteins – especially hemoglobin which accounts for approximately 97% of the cytosolic mass. The article states "Hemoglobin can also be depleted from an RBC lysate by employing Hemoglobind-
"It is worthwhile to note that the authors describe both our strategies for hemoglobin depletion, as the correct choice will vary with the application. With our own experience and with users such as those referenced in this article, we have gained the necessary knowledge to guide our users to the best option for hemoglobin depletion and/or low abundance enrichment" states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
References Acknowledged in the Review
 G. Alvarez-Llamas, F. de la Cuesta, M.G. Barderas, V.M. Darde, I. Zubiri, C. Caramelo, F. Vivanco. A novel methodology for the analysis of membrane and cytosolic sub-proteomes of erythrocytes by 2-DE Electrophoresis, 30 (2009), pp. 4095–4108
 K. Walpurgis, M. Kohler, A. Thomas, F. Wenzel, H. Geyer, W. Schanzer, M. Thevis. Validated hemoglobin-depletion approach for red blood cell lysate proteome analysis by means of 2D PAGE and Orbitrap MS Electrophoresis, 33 (2012), pp. 2537–2545
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HemoVoid™ - Hemoglobin Depletion From Erythrocytes
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Biotech Support Group LLC is a leading developer of proteomic, metabolomic and genomic sample preparation and enrichment products. It's principal products include: AlbuVoid™ & AlbuSorb™ for albumin depletion, Cleanascite™
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