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Follow on Google News | Synovial fluid biomarker discovery sample preparation utilizing Albusorb™ for albumin depletionAlbuSorb™ from Biotech Support Group for albumin depletion from synovial fluid samples to comparatively find and analyze novel biomarkers of rheumatoid arthritis of disease and non disease patient samples by proteomic techniques.
By: Biotech Support Group Proteomic biotechniques allow for the analysis of proteins in synovial fluid. After using Albusorb™, synovial fluid proteins on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is studied. Abundant proteins HSA and IgG are depleted using Albusorb™, followed by size-exclusion chromatography (SEC) of intact proteins prior to preparation of samples for analysis by LC/LC-MS/MS. Next tandem mass spectrometry (MS/MS), coupled with multidimensional liquid chromatography (LC) and database searching is effective technique for protein identification and characterization. Examples of elevated biomarker candidates which are elevated in erosive or nonerosive rheumatoid arthritis (RA) are G3PDH,Peptidylprolyl isomerase, Cystatin B, Phosphoglycerate mutase 1, α2-plasmin inhibitor, S100A8 (calgranulin A), IgG1H Nie, Thymosin-β4. The ultimate goal of such application would be to comparatively find and analyze novel biomarkers of rheumatoid arthritis and search for the same biomarkers in synovial fluid of disease and non disease patient samples by proteomic techniques. Characteristics of Albusorb™ Removes >90% albumin from 30 mg albumin/ml sample Affinity-type equivalence, virtually no cross-reactivity with other proteins Bind and elute procedure - simply weigh powder, condition the sample, centrifuge and/or filter, and recover the albumin depleted serum Economical new surface technology, not based on affinity chromatography Mild conditions maintain tertiary structure of proteins and simple transfer to secondary analysis The albumin depleted filtrate retains the enzymatic and biological activity Removes albumin from samples such serum, plasma and from species including human, mouse, sheep, bovine, goat, rat, and calf The flow through fraction is compatible with LC-MS, activity based protein profiling and proteomic studies. Biotech Support Group is interested in collaborating on research and development of peptide inhibitors of the complement system by using Albusorb™ for sample preparation. Biotech Support Group also has AlbuVoid™ for albumin depletion plus low abundance serum protein enrichment. For more information, visit: AlbuSorb™ Albumin Depletion Kit http://www.biotechsupportgroup.com/ AlbuVoid™ - Albumin Depletion and Low Abundance Protein Enrichment Kit from Serum or Plasma http://www.biotechsupportgroup.com/ About Biotech Support Group LLC Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuVoid™ CONTACT: Dr.Swapan Roy Biotech Support Group 1 Deer Park Drive, Suite M, Monmouth Junction, NJ 08852, USA 732-274-2866 sales@biotechsupportgroup.com Albusorb™ References: Gwenael Pottiez, Pawel Ciborowski. 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