Cleanascite™ Employed to Help Determine Role of Lipogenesis in Pulmonary Arterial Hypertension

By: Biotech Support Group
 
MONMOUTH JUNCTION, N.J. - Sept. 6, 2022 - PRLog -- Biotech Support Group reports on an article, describing the simplicity and efficiency of their lipid clearance sample preparation technology to help determine the role of lipogenesis in a cell response study of pulmonary arterial vascular smooth muscle cells. The citation is:

The study aimed to evaluate the status and role of lipogenesis in pulmonary arterial vascular smooth muscle cells (PAVSMC) proliferation and survival. The article states "To achieve the lipid-deprived cell culture condition, cell culture grade fetal bovine serum (FBS) and BSA were delipidated using Cleanascite …". The study found that lipid accumulation detected in PAH PAVSMC was preserved in media deprived from exogenous lipids, indicating that PAH PAVSMC have an ability to generate lipids de novo. Importantly, PAH PAVSMC, in contrast to control cells, demonstrated increased growth not only in serum-deprived media, but in lipid-deprived serum-deprived media. The authors conclude that these data demonstrate that human Pulmonary Hypertension PAVSMC have up-regulated lipogenesis, which is supported in an Akt- and glycolysis-dependent manner and is required for increased proliferation and survival.

"We now have over twenty references, showing that Cleanascite™ was used to help identify a characteristic feature of in vitro cell response. Unlike alternative lipid-depletion methods that use solvents, Cleanascite™ is an aqueous suspension product and so it is very compatible with cellular models of disease. This ultimately helps with investigations such as this, whereby the cells require fetal bovine serum for growth, but the exogenous lipids need to be removed as part of the lipid-depleted media control." states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

To download whitepaper entitled "Cleanascite™ - Lipid Removal and Cell Response Applications", visit:

https://www.biotechsupportgroup.com/v/vspfiles/templates/257/pdf/CleanasciteCellResponseReferenceApplications113021.pdf

For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm

For Business Development, contact: Matthew Kuruc 732-274-2866, mkuruc@biotechsupportgroup.com (mailto:sales@biotechsupportgroup.com)

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Tags:Hypertension
Industry:Biotech
Location:Monmouth Junction - New Jersey - United States
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