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Cleanascite™ Employed to Investigate Lipid Influence in Macrophage Maturation in Cancer
By: Biotech Support Group
Albakri, Marwah M., et al. "Fatty acids secreted from head and neck cancer induce M2‐like Macrophages (https://jlb.onlinelibrary.wiley.com/
Tumor-infiltrating monocytes can mature into Macrophages that support tumor survival or that display antitumor properties. To explore mechanisms steering Macrophage maturation, the authors assessed the effects of supernatants from squamous cell carcinoma cell lines (FaDu and SCC) on monocyte-derived Macrophage maturation. To assess depletion of fatty acids from tumor supernatants, tumor-conditioned medium was treated with Cleanascite according to the manufacturer's instructions and prior to incubation with monocytes. The article states "Depletion of Fatty acids with Cleanascite from FaDu or SCC supernatants largely reversed the phenotypic changes in Macrophages otherwise observed by incubating monocytes in these supernatants"
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