Cleanascite™ Employed to Investigate Lipid Influence in Macrophage Maturation in Cancer

By: Biotech Support Group
 
MONMOUTH JUNCTION, N.J. - March 17, 2022 - PRLog -- Biotech Support Group reports on an article, describing the simplicity and efficiency of their lipid clearance technology for depletion of lipids in monocyte cultures prepared with supernatants from cancer cell lines. The citation is:

Albakri, Marwah M., et al. "Fatty acids secreted from head and neck cancer induce M2‐like Macrophages (https://jlb.onlinelibrary.wiley.com/doi/10.1002/JLB.1A0521-251R)." Journal of Leukocyte Biology (2022).

Tumor-infiltrating monocytes can mature into Macrophages that support tumor survival or that display antitumor properties. To explore mechanisms steering Macrophage maturation, the authors assessed the effects of supernatants from squamous cell carcinoma cell lines (FaDu and SCC) on monocyte-derived Macrophage maturation. To assess depletion of fatty acids from tumor supernatants, tumor-conditioned medium was treated with Cleanascite according to the manufacturer's instructions and prior to incubation with monocytes. The article states "Depletion of Fatty acids with Cleanascite from FaDu or SCC supernatants largely reversed the phenotypic changes in Macrophages otherwise observed by incubating monocytes in these supernatants". Macrophages incubated with either palmitic acid or oleic acid developed similar phenotypes as cells incubated in tumor supernatants. The authors conclude that fatty acids are sufficient to mediate monocyte skewing towards M2-like cells.

"We now have over twenty references, many investigating cancer, showing that Cleanascite™ was used to help identify a characteristic feature of in vitro cell response. Unlike alternative lipid-depletion methods that use solvents, Cleanascite™ is an aqueous suspension product and so it is very compatible with cellular models of disease. This ultimately helps with investigations such as this, that need to determine whether or not lipids, or factors associated with lipids, impart phenotypic changes to cells." states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

To download whitepaper entitled "Cleanascite™ - Lipid Removal and Cell Response Applications", visit:

https://www.biotechsupportgroup.com/v/vspfiles/templates/257/pdf/CleanasciteCellResponseReferenceApplications113021.pdf

For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For more information, go to http://www.biotechsupportgroup.com.

For Business Development, contact: Matthew Kuruc 732-274-2866, mkuruc@biotechsupportgroup.com (mailto:sales@biotechsupportgroup.com)

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Tags:Cancer
Industry:Biotech
Location:Monmouth Junction - New Jersey - United States
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