Cleanascite™ Employed to Investigate Lipid Influences in Melasma

By: Biotech Support Group
MONMOUTH JUNCTION, N.J. - March 17, 2022 - PRLog -- Biotech Support Group reports on an article, describing the simplicity and efficiency of their lipid clearance sample preparation technology for studying the influence of lipids in Melasma using co-cultures with skin explants. The citation is:

Flori, E., Mastrofrancesco, A., Mosca, S., Ottaviani, M., Briganti, S., Cardinali, G., Filoni, A., Cameli, N., Zaccarini, M., Zouboulis, C.C., Picardo, M., Sebocytes contribute to melasma onset, ISCIENCE (2022), doi:

Melasma is a hyper-pigmentary disorder with photoaging features, whose manifestations appear on specific face areas, rich in sebaceous glands. To verify whether sebocyte-specific lipids could contribute to the observed changes in cell lines, the investigators tested lipid depleted irradiated SZ95 conditioned medium to treat these cells. For this, the BSG product Cleanascite™ was used. The article states: "Cleanascite was added to the collected SZ95 medium in a ratio 1:4 and mixed for 10 min at room temperature by gentle shaking. Following centrifugation (16000g) for 1 min at 4 °C, the supernatant was carefully decanted into a clean collection vial and used for experiments." The authors indicate sebocytes as one of the actors in melasma pathogenesis, inducing prolonged skin cells stimulation, contributing to localized dermal aging and hyperpigmentation.

"We have over twenty references showing that Cleanascite™ was used to help identify a characteristic feature of in vitro cell response. Unlike alternative lipid-depletion methods that use solvents, Cleanascite™ is an aqueous suspension product and so it is very compatible with cellular models of disease. This ultimately helps with investigations such as this, that need to determine whether or not lipids, or factors associated with lipids, impart phenotypic changes to cells." states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

To download whitepaper entitled "Cleanascite™ - Lipid Removal and Cell Response Applications", visit:

For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure.

For Business Development, contact: Matthew Kuruc 732-274-2866, (

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