EZClick™ EdU Cell Proliferation/DNA Synthesis Kit (FACS/Microscopy), Green

For detection of DNA synthesis in proliferating cells and assessment of the cell cycle phase.
MILPITAS, Calif. - Sept. 10, 2021 - PRLog -- All living cells undergo a cell division cycle, a biological process crucial for proliferation and inheritance. Cell-division cycle is a series of events resulting in two daughter cells containing replicas of DNA from the original DNA molecule. DNA replication occurs in the S phase of the cell cycle and involves de novo synthesis of genomic DNA from its precursors. The ability to monitor and obtain detailed characterization of the cell cycle and DNA synthesis in proliferating cells is fundamental in basic, and applied immunologic and oncologic studies. Accurate determination of the effect of biologically active reagents on DNA synthesis and cell cycle is of great importance in anti-cancer drug discovery and basic biology.

BioVision's EZClick™ EdU Proliferation/DNA Synthesis Kit utilizes a novel approach that relies on the incorporation of 5-EdU (5-ethynyl-2'deoxyuridine), a nucleoside analog to thymidine into the newly synthesized DNA directly in the cell culture. Incorporation of EdU into genomic DNA in S-phase is detected based on a click reaction between the alkyne moiety of the EdU and fluorescent azide.

Compared to historically used BrdU reactions, click reaction is carried out under mild conditions and flow cytometry/fluorescence microscopy can be used for the assessment of proliferating cells in the population. The kit provides sufficient materials for 100 assays based on the protocol below.

Figures: (A) Background Cells: Green fluorescence (middle upper panel) was not detected inside these cells (no Edu DNA Label, with fluorescent Azide). Positive Control Cells:Green fluorescence corresponding to the EZclick™ EdU DNA labeling (middle lower panel) were detectable after cells exposed to EdUDNA Labeling for 24 hr.

(B) Jurkat cells were cultured in the presence of 1X EdU DNA Labeling for 24 hr.at 37 °C and then processed for detection of de novo DNA synthesis. Green Fluorescence was measured in FL-1 channel in the linear mode.

Storage Conditions -20°C
USAGE For Research Use Only! Not For Use in Humans.
For more information on this Assay Kit Cat# K2070 https://bit.ly/3k0RVeJ

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Page Updated Last on: Sep 10, 2021

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