EZClick™ EdU Cell Proliferation/DNA Synthesis Kit (FACS/Microscopy), Green
For detection of DNA synthesis in proliferating cells and assessment of the cell cycle phase.
By: BioVision Inc
BioVision's EZClick™ EdU Proliferation/
Compared to historically used BrdU reactions, click reaction is carried out under mild conditions and flow cytometry/fluorescence microscopy can be used for the assessment of proliferating cells in the population. The kit provides sufficient materials for 100 assays based on the protocol below.
Figures: (A) Background Cells: Green fluorescence (middle upper panel) was not detected inside these cells (no Edu DNA Label, with fluorescent Azide). Positive Control Cells:Green fluorescence corresponding to the EZclick™ EdU DNA labeling (middle lower panel) were detectable after cells exposed to EdUDNA Labeling for 24 hr.
(B) Jurkat cells were cultured in the presence of 1X EdU DNA Labeling for 24 hr.at 37 °C and then processed for detection of de novo DNA synthesis. Green Fluorescence was measured in FL-1 channel in the linear mode.
Storage Conditions -20°C
USAGE For Research Use Only! Not For Use in Humans.
For more information on this Assay Kit Cat# K2070 https://bit.ly/
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Page Updated Last on: Sep 10, 2021