c-Jun Transcription Factor Activity Assay Kit (Colorimetric)

A 96-well plate based colorimetric assay to measure the activation of human or mouse AP-1/c-Jun in nuclear extracts or cell lysates.
MILPITAS, Calif. - Aug. 12, 2021 - PRLog -- Activator protein-1 (aka activating protein 1, AP-1) is a critical transcription factor that plays a central role in the regulation of genes important in many cellular processes including cell proliferation, differentiation, apoptosis , survial, migration etc.

AP-1 family is composed of homodimers and heterodimers of Jun, Fos, ATF or MAF protein families, which are characterized by the highly conserved dimeric basic leucine zipper (bZIP) DNA-binding domains. AP-1 protein is primarily regulated at the level of both Jun and Fos gene transcription involving MAPKs pathways and by post-translational modification via phosphorylation and dephosphorylation.

AP-1 activity is induced by either physiological stimuli or environmental insults, including TPA, growth factors, polypeptide hormones, cytokines, UV irradiation, bacterial or viral infections etc. BioVision's c-Jun Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of human or mouse c-Jun in nuclear extracts or cell lysates.

The kit offers an easy, rapid, sensitive and non-radioactive way to detect the activation of c-Jun in samples. In this assay, double stranded DNA sequence containing the c-Jun consensus binding site is coated on the 96-well plate.

Active c-Jun in the cell lysate or the nuclear extract binds to the oligonucleotides on the plate. After the addition of c-jun primary antibody that recognizes the c-Jun-oligonucleotide complex, a HRP-conjugated secondary antibody is added followed by the addition of TMB substrate and a color signal is developed, which is measured at 450 nm.

Figures. (A, B) Transcription factor activity assay using nuclear extracts from K562 cells treated with 12-O-Tetradecanoylphorbol-13-acetate (TPA). Assay was performed following the assay kit protocol.

Storage Conditions Multiple Temperatures
USAGE For Research Use Only! Not For Use in Humans.
For more information on this Assay Kit Cat# K2097 https://bit.ly/2VOQlDq

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