Indican Assay Kit (Colorimetric)

A simple, easy quantitative assay to determine the indican concentration in urine samples
MILPITAS, Calif. - May 3, 2021 - PRLog -- Indican, also known as indoxyl sulfate is a putrefaction product resulting from the bacterial deconjugation of dietary tryptophan in the small intestine. Most of the indican is excreted through feces, while the remainder is absorbed, metabolized and excreted in urine. Indican levels are directly associated with the bacterial activity in the intestine and its levels are low in normal urine. However, an increase in urine indican levels can indicate inefficient protein digestion such as bowel obstructions, stomach disorder, intestinal disorder, pancreatic insufficiency or overgrowth of the gut anaerobic bacteria. Thus, urinary indican levels can be a beneficial screening tool for dysbiosis, small intestine anaerobic bacterial overgrowth, malabsorption, maldigestion, intestinal mucosal permeability etc.

BioVision's Indican Assay Kit is a quantitative assay to determine the indican concentration in urine samples. In this assay, indican reacts with p-dimethylaminobenzaldehyde (DMAB) in the presence of an acid to yield an orange-colored compound measured at 480 nm. The intensity of the color is directly proportional to the indican concentration in the sample(s). The assay is simple, easy to perform and is reliable. The assay detection range is between 0.5-30 mg/dl.

Figures: (a) Indican Standard Curve. (b) Indican amounts (mg/dl) in human urine samples (50 μl). Calculation is based on samples spiked with 5 mg/dl Indican Standard. (c) Results were normalized as mg of Indican per mg creatinine by using Creatinine Colorimetric/Fluorometric Assay Kit (BioVision Cat# K625-100). Assays were performed following the kit protocol.

For more information on this assay kit Cat# K2083, Visit:

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