Journal Article Cites HemoVoid™ and AlbuVoid™ Enrichment for LC-MS Proteomic Analysis
By: Biotech Support Group
The citation is:
Jing, Lun, et al. "PROTEOMIC ANALYSIS IDENTIFIED LBP AND CD14 AS KEY PROTEINS IN BLOOD/BIPHASIC CALCIUM PHOSPHATE MICROPARTICLE INTERACTIONS (https://www.sciencedirect.com/
Immediately upon implantation, scaffolds for bone repair are exposed to the patient's blood. Biphasic calcium phosphate (BCP) ceramics are considered as the gold standard in bone reconstruction surgery. Here, in a LC-MS/MS proteomic study, the authors compared the differentially expressed blood proteins (plasma and blood cell proteins) and the deregulated signaling pathways of osteogenic and fibrogenic blood composites. The article describes use of HemoVoid™ for depletion of Hemoglobin prior to LC-MS analysis, "each composite material or 4 blood clots were pooled into 3ml of cooled lysis buffer [HEPES 50mM (pH 7.4); NaCl 150mM; EDTA 20mM (pH 8); CHAPS 1%; DTT 1mM; Protease and Phosphatase inhibitor cocktail]. They were let on ice for 30min with regular vortexings and centrifuged at 4°C, 8000g, 15 min. The supernatants were collected, and hemoglobin depleted using several HemoVoid™ columns (Biotech Support Group). When indicated, albumin was also partially removed using AlbuVoid™ depletion reagent kit (Biotech Support Group) following the manufacturer's instructions."
"Here is an excellent report demonstrating a very important advantage of the variety of sample prep products that we supply. ", states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
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