Nicotinamide N-methyltransferase Inhibitor Screening Kit II (F)
A one step, non-coupled, plate-based fluorometric assay for screening potential inhibitors of NNMT.
By: BioVision, Inc.
BioVision's Nicotinamide N-methyltransferase inhibitor screening Kit II is a non-coupled, plate-based fluorometric assay. The assay utilizes a selective NNMT substrate that is methylated by SAM to generate a fluorescent product, measured at Ex/Em = 320/420 nm. In the presence of 1-Methylnicotinamide, a NNMT inhibitor, the enzymatic activity is inhibited thereby resulting in a reduced fluorescent signal. The kit provids a one-step, rapid, reliable test for high-throughput screening of NNMT inhibitors.
Figures. a) NNMT enzyme activity in the presence or absence of MNA. b) NNMT enzyme activity in the presence or absence of DMSO. c) Inhibition of NNMT enzyme activity by MNA. IC50 of MNA was calculated as 25.45±2.5 μM. Assays were performed following the kit protocol.
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