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Sulfated Glycosaminoglycans Assay Kit
A simple, rapid, sensitive and convenient way to measure the amount of sulfated GAGs in samples.
By: BioVision, Inc.
Additionally, GAGs play an important role in many biological processes including cell signaling, cell growth and proliferation, anti-coagulation, angiogenesis, tumor progression, etc. GAGs are degraded in the lysosome via four different pathways. However, patients with the lysosomal storage disorder known as mucopolysaccharidoses (MPSs) have impaired lysosomal break down of GAGs and hence have higher levels of GAGs in their tissues or urine. Abnormal accumulation of GAGs in cells, blood and connective tissues over time can lead to permanent cell damage that can affect appearance, movement and functioning of organs.
BioVision's Sulfated-Glycosaminoglycans Assay Kit efficiently detects all sulfated GAGs including heparan sulfate, chondroitin sulfate and keratan sulfate in various biological samples. In this assay, a cationic dye, 1,9-dimethylmethlene Blue (DMMB) specifically interacts with the highly negatively charged sulfated GAGs to produce a colored product, which is measured at absorbance 525 nm. The colored signal is directly proportional to the concentration of sulfated GAGs in the sample. The kit offers a simple, rapid, sensitive and convenient way to measure the amount of sulfated GAGs in samples. It can detect as low as 0.5 μg of sulfated GAGs under the assay conditions.
Figures. A. S-GAG Standard Curve. B. Concentration of S-GAGs in unspiked and spiked urine samples. > 95% recovery was observed when spiked with 10 μg/ml S-GAG. C. Amount of S-GAGs in rat muscles. Assay was performed according to the assay protocol.
For more information on this Assay Kit Cat# K2074; Visit: https://bit.ly/
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