PNGase F Deglycosylation Assay Kit

MILPITAS, Calif. - Dec. 8, 2020 - PRLog -- Peptide-N4-(N-acetyl-β-D-glucosiminyl asparagine amidase F (PNGase F) is an amidohydrolase that cleaves the N-glycan chains at the innermost N-Acetylglucosamine (GlcNAc) of glycans and asparagine residues of high mannose, hybrid and complex oligosaccharides of N-linked glycoproteins and glycopeptides. The cleavage results in a deaminated protein or peptide and free glycans. PNGase F is widely used in the deglycosylation of glycoproteins to study glycans and identify glycosylation sites.

BioVision's PNGase F Deglycosylation Kit is designed for the quick deglycosylation of glycoproteins using PNGase F under optimized conditions. The kit provides all the necessary reagents for the deglycosylation at least 20 mg of glycoproteins.

Figure A. 20 µg of various glycoproteins were deglycosylated using 0.1 mU of PNGase F (BioVision Cat# P1598) for 1 hr at 37 °C. The deglycosylation was monitored on a 4-20% gradient SDS-PAGE. 3 µg of Ovalbumin; Recombinant Sars-Cov-2 Spike protein 1 (BV Cat# P1653); Recombinant Sars-Cov-2 Spike protein 1 receptor-binding domain (RBD-Fc) (BV Cat# P1654 ) and RNase B were used for the deglycosylation studies. D: Deglycosylated glycoprotein. G: Glycoprotein.

For more information on this Assay Kit Cat# K2061, Visit: https://bit.ly/3gqe6aN