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Follow on Google News | Sialyltransferase Activity Assay Kit (Fluorometric)For the measurement of Sialyltransferase activity using a 96-well plate format
By: BioVision Inc BioVision's Sialyltransferase Activity Assay Kit is a simple, two-step plate-based assay that measures the fluorescent product at Ex/Em = 410/470 nm. The assay consists of three enzymatic steps leading to the generation of ammonia. Ammonia then reacts with the detection reagent to form a fluorescent product measured at Ex/Em = 410/470 nm. The kit can detect activity from both α-1,6 and α-2,6 sialyltransferases and can measure as low as 20 μU ST activity under assay conditions. Figures: A). NH4Cl Standard Curve. B). Fluorescence signal obtained for α-2,3 and α-2,6 ST sialyltransferases. C). ST specific activity in brain and liver lysates. Experiments were conducted according to the kit protocol. For more information on this Assay Kit, visit: https://www.biovision.com/ End
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